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AT1G77760.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
cytosol 1.000
endoplasmic reticulum 0.000
ASURE: cytosol,nucleus
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:29662028 (2018): nucleus nuclear matrix
  • PMID:29662028 (2018): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : nitrate reductase 1
Curator
Summary (TAIR10)
Encodes the cytosolic minor isoform of nitrate reductase (NR). Involved in the first step of nitrate assimilation, it contributes about 15% of the nitrate reductase activity in shoots. Similar to molybdopterin oxidoreductases at the N-terminus, and to FAD/NAD-binding cytochrome reductases at the C-terminus. Cofactors: FAD, heme iron (cytochrome B-557), and molybdenum-pterin.
Computational
Description (TAIR10)
nitrate reductase 1 (NIA1); FUNCTIONS IN: nitrate reductase activity, protein binding; INVOLVED IN: nitric oxide biosynthetic process, nitrate assimilation, response to light stimulus; LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome b5, heme-binding site (InterPro:IPR018506), Nitrate reductase NADH dependent (InterPro:IPR012137), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Immunoglobulin E-set (InterPro:IPR014756), Cytochrome b5 (InterPro:IPR001199), NADH:cytochrome b5 reductase (CBR) (InterPro:IPR001834), Oxidoreductase, molybdopterin-binding domain (InterPro:IPR000572), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Oxidoreductase, molybdopterin binding site (InterPro:IPR022407), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Eukaryotic molybdopterin oxidoreductase (InterPro:IPR008335), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Moybdenum cofactor oxidoreductase, dimerisation (InterPro:IPR005066); BEST Arabidopsis thaliana protein match is: nitrate reductase 2 (TAIR:AT1G37130.1); Has 14813 Blast hits to 14437 proteins in 2198 species: Archae - 168; Bacteria - 6976; Metazoa - 1728; Fungi - 2215; Plants - 1479; Viruses - 3; Other Eukaryotes - 2244 (source: NCBI BLink).
Protein Annotations
BioCyc:MetaCyc:AT1G77760-MONOMERBioGrid:29331EC:1.7.1.1eggNOG:COG0543
eggNOG:COG2041eggNOG:COG5274eggNOG:ENOG410JWE3eggNOG:KOG0534
eggNOG:KOG0535eggNOG:KOG0537EMBL:AC012193EMBL:AF424624
EMBL:AY090950EMBL:BT000989EMBL:CP002684EMBL:X13434
EMBL:X13436EMBL:Z19050EnsemblPlants:AT1G77760EnsemblPlants:AT1G77760.1
entrez:844112Gene3D:2.60.40.650Gene3D:3.10.120.10Gene3D:3.90.420.10
GeneID:844112Genevisible:P11832GO:GO:0005829GO:GO:0006809
GO:GO:0008940GO:GO:0009416GO:GO:0009635GO:GO:0009703
GO:GO:0020037GO:GO:0030151GO:GO:0042128GO:GO:0043546
GO:GO:0050464Gramene:AT1G77760.1hmmpanther:PTHR19370hmmpanther:PTHR19370:SF100
HOGENOM:HOG000252609InParanoid:P11832IntAct:P11832InterPro:IPR000572
InterPro:IPR001199InterPro:IPR001433InterPro:IPR001709InterPro:IPR001834
InterPro:IPR005066InterPro:IPR008333InterPro:IPR008335InterPro:IPR012137
InterPro:IPR014756InterPro:IPR017927InterPro:IPR017938InterPro:IPR018506
InterPro:IPR022407iPTMnet:P11832KEGG:ath:AT1G77760KO:K10534
OMA:QESAWIVPaxDb:P11832Pfam:P11832Pfam:PF00173
Pfam:PF00174Pfam:PF00175Pfam:PF00970Pfam:PF03404
Pfscan:PS50255Pfscan:PS51384PhylomeDB:P11832PIR:E96807
PIR:S35228PIRSF:PIRSF000233PRIDE:P11832PRINTS:PR00363
PRINTS:PR00371PRINTS:PR00406PRINTS:PR00407PRO:PR:P11832
PROSITE:PS00191PROSITE:PS00559PROSITE:PS50255PROSITE:PS51384
ProteinModelPortal:P11832Proteomes:UP000006548Reactome:R-ATH-196836RefSeq:NP_177899.1
scanprosite:PS00191scanprosite:PS00559SMART:SM01117SMR:P11832
STRING:3702.AT1G77760.1SUPFAM:SSF52343SUPFAM:SSF55856SUPFAM:SSF56524
SUPFAM:SSF63380SUPFAM:SSF81296TAIR:AT1G77760tair10-symbols:GNR1
tair10-symbols:NIA1tair10-symbols:NR1UniGene:At.17771UniProt:P11832
Coordinates (TAIR10) chr1:-:29236005..29239367
Molecular Weight (calculated) 103046.00 Da
IEP (calculated) 6.79
GRAVY (calculated) -0.37
Length 917 amino acids
Sequence (TAIR10)
(BLAST)
001: MATSVDNRHY PTMNGVAHAF KPPLVPSPRS FDRHRHQNQT LDVILTETKI VKETEVITTV VDSYDDSSSD DEDESHNRNV PYYKELVKKS NSDLEPSILD
101: PRDESTADSW IQRNSSMLRL TGKHPFNAEA PLPRLMHHGF ITPVPLHYVR NHGAVPKANW SDWSIEITGL VKRPAKFTME ELISEFPSRE FPVTLVCAGN
201: RRKEQNMVKQ TIGFNWGSAG VSTSLWKGIP LSEILRRCGI YSRRGGALNV CFEGAEDLPG GGGSKYGTSI KKEMAMDPAR DIILAYMQNG ELLTPDHGFP
301: VRVIVPGFIG GRMVKWLKRI IVTPQESDSY YHYKDNRVLP SLVDAELANS EAWWYKPEYI INELNINSVI TTPGHAEILP INAFTTQKPY TLKGYAYSGG
401: GKKVTRVEVT LDGGDTWSVC ELDHQEKPNK YGKFWCWCFW SLDVEVLDLL SAKDVAVRAW DESFNTQPDK LIWNLMGMMN NCWFRIRTNV CKPHRGEIGI
501: VFEHPTRPGN QSGGWMAKER QLEISSESNN TLKKSVSSPF MNTASKMYSI SEVRKHNTAD SAWIIVHGHI YDCTRFLKDH PGGTDSILIN AGTDCTEEFE
601: AIHSDKAKKL LEDYRIGELI TTGYDSSPNV SVHGASNFGP LLAPIKELTP QKNIALVNPR EKIPVRLIEK TSISHDVRKF RFALPSEDQQ LGLPVGKHVF
701: VCANINDKLC LRAYTPTSAI DAVGHIDLVV KVYFKDVHPR FPNGGLMSQH LDSLPIGSMI DIKGPLGHIE YKGKGNFLVS GKPKFAKKLA MLAGGTGITP
801: IYQIIQSILS DPEDETEMYV VYANRTEDDI LVREELEGWA SKHKERLKIW YVVEIAKEGW SYSTGFITEA VLREHIPEGL EGESLALACG PPPMIQFALQ
901: PNLEKMGYNV KEDLLIF
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)