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AT4G22970.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
nucleus 1.000
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : homolog of separase
Curator
Summary (TAIR10)
Encodes a separase (ESP), homologous to human and mouse separase protein. Separase is a capase family protease required for the release of sister chromatid cohesion during meiosis and mitosis. Arabidopsis separase contains a predicted 2Fe2S-ferredoxin domain that is not present in the proteins of other organisms. Also contains a putative EF-hand calcium binding domain. Mutant seeds exhibited embryo arrest at the globular stage. The endosperm also exhibited a weak titan-like phenotype. Transgenic plants expressing AESP RNA interference (RNAi) from the meiosis-specific DMC1 promoter exhibited alterations in chromosome segregation during meiosis I and II that resulted in polyads containing from one to eight microspores. Plays an essential role in embryo development. Required for the removal of cohesin from meiotic chromosomes and establishment of meiotic nuclear domains. This gene was also identified through the rsw4 mutant. Lines carrying recessive, temperature-sensitive mutations exhibit reduced anisotropic growth at 30 degrees Celsius. Microtubules and cellulose microfibrils are not depleted or disoriented in the mutants at the restrictive temperature.
Computational
Description (TAIR10)
homolog of separase (ESP); FUNCTIONS IN: peptidase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase C50, separase (InterPro:IPR005314); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28550.1); Has 401 Blast hits to 372 proteins in 177 species: Archae - 0; Bacteria - 2; Metazoa - 98; Fungi - 137; Plants - 87; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT4G22970-MONOMERBioCyc:ARA:GQT-1296-MONOMERBioGrid:13685BRENDA:3.4.22.49
EC:3.4.22.49eggNOG:COG5155eggNOG:KOG1849EMBL:AL031018
EMBL:AL161558EMBL:AY823256EMBL:CP002687EnsemblPlants:AT4G22970
EnsemblPlants:AT4G22970.1entrez:828396Gene3D:1.25.40.10GeneID:828396
Genevisible:Q5IBC5GO:GO:0000911GO:GO:0004197GO:GO:0005634
GO:GO:0005737GO:GO:0005874GO:GO:0006887GO:GO:0007067
GO:GO:0008233GO:GO:0009793GO:GO:0009826GO:GO:0009960
GO:GO:0016192GO:GO:0045876GO:GO:0051304GO:GO:0051307
GO:GO:0072686GO:GO:1901970GO:GO:2000114hmmpanther:PTHR12792
HOGENOM:HOG000138034InParanoid:Q5IBC5InterPro:IPR005314InterPro:IPR011990
InterPro:IPR030397iPTMnet:Q5IBC5KEGG:ath:AT4G22970KO:K02365
MEROPS:C50.005ncoils:CoilOMA:ELCCQTIPANTHER:PTHR12792
PaxDb:Q5IBC5Pfam:PF03568Pfam:Q5IBC5Pfscan:PS51700
PhylomeDB:Q5IBC5PIR:T05128PRIDE:Q5IBC5PRO:PR:Q5IBC5
PROSITE:PS51700ProteinModelPortal:Q5IBC5Proteomes:UP000006548Reactome:R-ATH-2467813
RefSeq:NP_001190804.1RefSeq:NP_194028.2STRING:3702.AT4G22970.1TAIR:AT4G22970
tair10-symbols:AESPtair10-symbols:ESPtair10-symbols:RSW4UniGene:At.32480
UniProt:Q5IBC5
Coordinates (TAIR10) chr4:-:12033703..12043572
Molecular Weight (calculated) 244860.00 Da
IEP (calculated) 7.14
GRAVY (calculated) -0.19
Length 2180 amino acids
Sequence (TAIR10)
(BLAST)
0001: MASSGDDLRL LSLIDVGDNV FSSFSDYLKP FSTLSTSRKK QDRATTIRAL AKQFLPFLNK SISLLPKRLS VANSDKEARE SALDLFRAYE LCLDCLELVS
0101: AQLACKPHTV QSQRLRMIHC LDVWGLYENV YTEAFKVLEK LRGSDSKSRK SRLLPEVQDG DAEMALVVVD AVAAIFRAVA MSQQLDDKRY RKVLLLLEEV
0201: GGWLRVLDAK VYEKLHRAMV TSMGKCAVSL VREAERFNGD LVISFCDLTV KEHYKSALSK DRVYKFAREV LSVLFGFKDR KMSVTIDISM SVLRSLSCQF
0301: EDESNENLME FFDLVDYCAH KFRAAGDMYC AKVSKKLNEM AAIFVEAIPQ LNLVLRLYST GLSITVCNSK LGEIKLEDST DDWKIQAMFD DDARWQSLVS
0401: LLGMVDSYSG DEGNQTGSSS IGGHRNYNNK THDSCKDRNK ITCWPQYVDA LKFLCQPLAD FIYSVKRKIV LETEMSCASA HLITIHDAFL QFCDGCLFLQ
0501: RCTSDKGDRE IANNKAFLNA AMGAFIVSLR TQLKLEISAH LVEDVIGSPW IQSQELKYLI ATLYNIGIVL YRNKELNKAC EALKLCSKVS WRCVELHCHM
0601: FVNQSSSSDN DLSEDAIMDF VGEACNRCAF YLDILQKCSR RKIRQNIVHI LENWLSAEHL IRRLPGPEAI VKQWVKIERE CHTDLDAAGS CTTLYSLLSS
0701: SQKKSKRGIG KILEQELLAY DRVLPLRSNL GQQTRIKIAD ILLKDVYVTE DMHIERARIL IWKARMTRTS GTEHITECIC FLSEAISILG ELHHGPNEEG
0801: SPSSHMLPIA YCLRAFCTQE ADPNSKKVFQ DISTSLNLWL RILSLDDSGD SLPTENIIPL LYNMIDLMSV KGCTELHHHI YQLIFRLFKW KNVKLEVCLA
0901: MLWECRRLSH ALCPSPISDA FIQTLSENCA DKSTCIDFWM DCLKDSKAKL IGFQQNFHDL HNKDEGPFQS DITIDDIKDA ASELISSASL SGNSSFAAAY
1001: LYYDLCERLI SFGKLSEALS YAKEAYRIRT LIFQDKFKYT AEKHIEKHNE DGKISEIRTF SIKNFQVYRL LATDFWPCGN FLWDINRCYL SPWSVLQCYL
1101: ESTLQVGILN ELIGNGLEAE TILSWGKAFS CSQSLFPFVV AFSSALGNLY HKKQCLDLAE KELQNAKEIL IANQRDFSCV KCKLKLEVTL DKQLGDISRK
1201: QIDRVSQTDG FLHAESLFSA ALGKFCCSAW KSCIRSHGEE IAEEIVIDRN GGEGLGHNSS KTKLSIKEPP GNRGSRRGGR ANKTCLSKDQ DLISEPTSRL
1301: TRSMRHSLRE QCQNRSNVPE VVSKKPNLCD RSVGSRGERV LLDTSNALPG FCICYKEKRQ QCLSEEVTES GSLNNLVSLK WELCHRKLAS SILVSLGKCL
1401: GDSGRIHLAH EALLHSISVL FKSTWSSHNQ PSVSQLLEFI GKEVTRDVFA VDRAIILYNL CWLNLRNYHC RKSRSICCDL FHIPFTKLVS WLMLAFVLSG
1501: EVPILFQKVS RLLASLYLLS SSNSEFTFES DGNELSASHW VSFFHQASLG THLSYHFISN LSQKHKSQCL SDKECTEATC SSCMVPEDLD LPRLAPDRTQ
1601: DLVQFAKEFF INLPSSTIIC ISLLGGALNQ LLQELMHIRS PVCAWVLISR LNPESQPVAT LLPVDSIVED MSDDSANLSS TEATQVKSLK GPWLCPWGTT
1701: VVDEVAPAFK SILEESHSSS STTEEDTIES RGLWWKKRKK LNHRLGIFLR NLEASWLGPW RCLLLGEWSN YKLPDSAQKK LVNDLKSKCK MEVNEMLLKV
1801: ILGGGTDNFK GEACVAQLSL RNGCYVGRGG YLYEEDSCKT PTAASNISES RHELALKLIH DAASKLGQQD GHENREPIIL VLDPEVQMLP WENIPILRKQ
1901: EVYRMPSVGC ISAVLKKRSL QGEPAKSHVA SFPLIDPLDS FYLLNPGGDL TDTQVTFESW FRDQNFEGKA GSEPSAIELT EALETHDLFL YFGHGSGAQY
2001: IPRREIEKLD NCSATFLMGC SSGSLWLKGC YIPQGVPLSY LLGGSPAIVA TLWDVTDRDI DRFGKALLEA WLQERSDSSS EGGCSQCESL ANDLAAMTLK
2101: GTKRSRKPSS RNKPAQSDVD GSGKIECNHK HRRKIGSFIA AARDACNLQY LIGAAPVCYG VPTGITRKKG IDALLPSSSR
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)