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AT3G30180.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
endoplasmic reticulum 0.998
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : brassinosteroid-6-oxidase 2
Curator
Summary (TAIR10)
Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control.
Computational
Description (TAIR10)
brassinosteroid-6-oxidase 2 (BR6OX2); FUNCTIONS IN: monooxygenase activity, oxygen binding; INVOLVED IN: response to light stimulus, circadian rhythm, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: brassinosteroid-6-oxidase 1 (TAIR:AT5G38970.1); Has 29372 Blast hits to 29308 proteins in 1622 species: Archae - 48; Bacteria - 4489; Metazoa - 10449; Fungi - 5456; Plants - 7735; Viruses - 3; Other Eukaryotes - 1192 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT3G30180-MONOMERBioCyc:MetaCyc:AT3G30180-MONOMERBioGrid:8036EC:1.14.-.-
eggNOG:COG2124eggNOG:KOG0157EMBL:AB087801EMBL:AP002060
EMBL:AY052655EMBL:AY063728EMBL:AY084595EMBL:CP002686
EnsemblPlants:AT3G30180EnsemblPlants:AT3G30180.1entrez:822709Gene3D:1.10.630.10
GeneID:822709Genevisible:Q940V4GO:GO:0004497GO:GO:0005506
GO:GO:0007275GO:GO:0010268GO:GO:0016021GO:GO:0016125
GO:GO:0016132GO:GO:0016705GO:GO:0020037Gramene:AT3G30180.1
gramene_pathway:1.14.13.-gramene_pathway:2.1.1.-gramene_pathway:2.7.7.-gramene_pathway:PWY-6544
gramene_pathway:PWY-699hmmpanther:PTHR24286hmmpanther:PTHR24286:SF8HOGENOM:HOG000237614
InParanoid:Q940V4InterPro:IPR001128InterPro:IPR002401InterPro:IPR017972
iPTMnet:Q940V4KEGG:ath:AT3G30180KO:K12640OMA:VQARNNI
PaxDb:Q940V4Pfam:PF00067Pfam:Q940V4PhylomeDB:Q940V4
PRIDE:Q940V4PRINTS:PR00385PRINTS:PR00463PRO:PR:Q940V4
PROSITE:PS00086ProteinModelPortal:Q940V4Proteomes:UP000006548Reactome:R-ATH-211916
Reactome:R-ATH-5365859RefSeq:NP_566852.1scanprosite:PS00086SMR:Q940V4
STRING:3702.AT3G30180.1SUPFAM:SSF48264TAIR:AT3G30180tair10-symbols:BR6OX2
tair10-symbols:CYP85A2TMHMM:TMhelixUniGene:At.26493UniPathway:UPA00381
UniProt:Q940V4
Coordinates (TAIR10) chr3:+:11810867..11813509
Molecular Weight (calculated) 53817.90 Da
IEP (calculated) 8.24
GRAVY (calculated) -0.25
Length 465 amino acids
Sequence (TAIR10)
(BLAST)
001: MGIMMMILGL LVIIVCLCTA LLRWNQMRYS KKGLPPGTMG WPIFGETTEF LKQGPDFMKN QRLRYGSFFK SHILGCPTIV SMDAELNRYI LMNESKGLVA
101: GYPQSMLDIL GTCNIAAVHG PSHRLMRGSL LSLISPTMMK DHLLPKIDDF MRNYLCGWDD LETVDIQEKT KHMAFLSSLL QIAETLKKPE VEEYRTEFFK
201: LVVGTLSVPI DIPGTNYRSG VQARNNIDRL LTELMQERKE SGETFTDMLG YLMKKEDNRY LLTDKEIRDQ VVTILYSGYE TVSTTSMMAL KYLHDHPKAL
301: EELRREHLAI RERKRPDEPL TLDDIKSMKF TRAVIFETSR LATIVNGVLR KTTHDLELNG YLIPKGWRIY VYTREINYDT SLYEDPMIFN PWRWMEKSLE
401: SKSYFLLFGG GVRLCPGKEL GISEVSSFLH YFVTKYRWEE NGEDKLMVFP RVSAPKGYHL KCSPY
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)