AT2G47460.1
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Subcellular Consensus
(Prediction and Experimental) min:  :max.
SUBAcon:nucleus 1.000 ASURE: nucleus What is SUBAcon? |
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| Experimental Localisations and PPI |
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SUBAcon links
AGI-AGI relationships |
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| Description (TAIR10) | protein_coding : myb domain protein 12 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Curator Summary (TAIR10) |
MYB12 belongs to subgroup 7 of the R2R3-MYB family. It strongly activates the promoters of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and - to a lesser extent - chalcone flavanone isomerase (CHI), but cannot activate the promoters of flavonoid-3'hydroxylase (F3'H) and dihydroflavonol 4-reductase (DF). The activation requires a functional MYB recognition element (MRE). Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. Overexpression or knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol), while seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX lines, but no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy. The redundant factors are MYB11 and MYB111 although MYB12 is primarily required for flavonol biosynthesis in roots. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Computational Description (TAIR10) |
myb domain protein 12 (MYB12); CONTAINS InterPro DOMAIN/s: SANT, DNA-binding (InterPro:IPR001005), Homeodomain-like (InterPro:IPR009057), Myb, DNA-binding (InterPro:IPR014778), HTH transcriptional regulator, Myb-type, DNA-binding (InterPro:IPR017930), Homeodomain-related (InterPro:IPR012287), Myb transcription factor (InterPro:IPR015495); BEST Arabidopsis thaliana protein match is: myb domain protein 11 (TAIR:AT3G62610.1); Has 8833 Blast hits to 8133 proteins in 470 species: Archae - 0; Bacteria - 0; Metazoa - 786; Fungi - 423; Plants - 5965; Viruses - 6; Other Eukaryotes - 1653 (source: NCBI BLink). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Protein Annotations |
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| Coordinates (TAIR10) | chr2:+:19476438..19479242 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Molecular Weight (calculated) | 41395.90 Da | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| IEP (calculated) | 4.86 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GRAVY (calculated) | -0.77 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Length | 371 amino acids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sequence (TAIR10) (BLAST) |
001: MGRAPCCEKV GIKRGRWTAE EDQILSNYIQ SNGEGSWRSL PKNAGLKRCG KSCRLRWINY LRSDLKRGNI TPEEEELVVK LHSTLGNRWS LIAGHLPGRT 101: DNEIKNYWNS HLSRKLHNFI RKPSISQDVS AVIMTNASSA PPPPQAKRRL GRTSRSAMKP KIHRTKTRKT KKTSAPPEPN ADVAGADKEA LMVESSGAEA 201: ELGRPCDYYG DDCNKNLMSI NGDNGVLTFD DDIIDLLLDE SDPGHLYTNT TCGGDGELHN IRDSEGARGF SDTWNQGNLD CLLQSCPSVE SFLNYDHQVN 301: DASTDEFIDW DCVWQEGSDN NLWHEKENPD SMVSWLLDGD DEATIGNSNC ENFGEPLDHD DESALVAWLL S |
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| See Also |
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Citation
If you find this resource useful please cite one of the following publications:
Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)
Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)