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AT4G20260.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
plasma membrane 1.000
ASURE: plasma membrane
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31818904 (2020): mitochondrion
  • PMID:31541795 (2020): plasma membrane
  • PMID:31520498 (2020): mitochondrion
  • PMID:31186290 (2019): cytosol None
  • PMID:30962257 (2019): plastid
  • PMID:30875866 (2019): plasma membrane
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:30447334 (2019): plasma membrane
  • PMID:30135097 (2018): plastid
  • PMID:29104584 (2017): nucleus nuclear matrix nucleolus
  • PMID:28524096 (2017): mitochondrion
  • PMID:27943495 (2017): mitochondrion
  • PMID:27936405 (2017): plasma membrane
  • PMID:27137770 (2016): plastid plastid stroma
  • PMID:26781341 (2016): plasma membrane
  • PMID:26572690 (2016): extracellular region plant-type cell wall
  • PMID:25900983 (2015): Golgi trans-Golgi network multivesicular body
  • PMID:25900983 (2015): Golgi trans-Golgi network
  • PMID:25900983 (2015): Golgi Golgi apparatus Golgi membrane
  • PMID:25900983 (2015): Golgi
  • PMID:25900983 (2015): plant-type vacuole plant-type vacuole membrane
  • PMID:25641898 (2015): plasma membrane
  • PMID:24397334 (2014): plasma membrane
  • PMID:24361574 (2014): plastid plastid stroma
  • PMID:24124904 (2013): plastid
  • PMID:23673981 (2013): plastid plastid stroma plastoglobules
  • PMID:23549413 (2013): plastid
  • PMID:23323832 (2013): plasma membrane
  • PMID:22923678 (2012): plasma membrane
  • PMID:22318864 (2012): plasma membrane
  • PMID:22215637 (2012): plasma membrane
  • PMID:20843791 (2010): plasma membrane
  • PMID:20374526 (2010): plasma membrane
  • PMID:20061580 (2010): plastid plastid envelope
  • PMID:19334764 (2009): plasma membrane
  • PMID:18998720 (2009): extracellular region
  • PMID:18686298 (2008): plant-type vacuole plant-type vacuole membrane
  • PMID:17644812 (2007): plasma membrane
  • PMID:17317660 (2007): plasma membrane
  • PMID:16635983 (2006): plasma membrane
  • PMID:15060130 (2004): plasma membrane
  • PMID:15028209 (2004): plastid
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : plasma-membrane associated cation-binding protein 1
Curator
Summary (TAIR10)
Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
Computational
Description (TAIR10)
plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, chloroplast, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 6213 Blast hits to 3707 proteins in 537 species: Archae - 35; Bacteria - 951; Metazoa - 1704; Fungi - 697; Plants - 277; Viruses - 59; Other Eukaryotes - 2490 (source: NCBI BLink).
Protein Annotations
BioGrid:13065eggNOG:ENOG410IYJBeggNOG:ENOG410YIYHEMBL:AF083669
EMBL:AK221216EMBL:AK317524EMBL:AL022224EMBL:AL161552
EMBL:AY086004EMBL:AY093084EMBL:AY128768EMBL:CP002687
EMBL:Y08061EnsemblPlants:AT4G20260EnsemblPlants:AT4G20260.1EnsemblPlants:AT4G20260.2
EnsemblPlants:AT4G20260.3entrez:827773ExpressionAtlas:Q96262GeneID:827773
Genevisible:Q96262GO:GO:0005507GO:GO:0005509GO:GO:0005516
GO:GO:0005546GO:GO:0005547GO:GO:0005881GO:GO:0005886
GO:GO:0006499GO:GO:0008017GO:GO:0009414GO:GO:0009506
GO:GO:0010350GO:GO:0016032GO:GO:0031117GO:GO:0035865
GO:GO:0043325GO:GO:0043622GO:GO:0046658GO:GO:0051511
GO:GO:0071219GO:GO:0071280GO:GO:0071281GO:GO:0071286
GO:GO:0071325GO:GO:0071472GO:GO:0072709GO:GO:0080025
hmmpanther:PTHR38522hmmpanther:PTHR38522:SF2HOGENOM:HOG000070951InParanoid:Q96262
InterPro:IPR008469iPTMnet:Q96262KEGG:ath:AT4G20260OMA:PEGKANP
PANTHER:PTHR38522PaxDb:Q96262Pfam:PF05558Pfam:Q96262
PhylomeDB:Q96262PIR:T05334PRIDE:Q96262PRO:PR:Q96262
ProMEX:Q96262ProteinModelPortal:Q96262Proteomes:UP000006548RefSeq:NP_001031676.1
RefSeq:NP_001031677.1RefSeq:NP_193759.1RefSeq:NP_849412.1STRING:3702.AT4G20260.4
SwissPalm:Q96262TAIR:AT4G20260tair10-symbols:ATPCAP1tair10-symbols:PCAP1
UniGene:At.21638UniGene:At.24152UniProt:Q96262
Coordinates (TAIR10) chr4:+:10941593..10943227
Molecular Weight (calculated) 24585.00 Da
IEP (calculated) 4.65
GRAVY (calculated) -0.71
Length 225 amino acids
Sequence (TAIR10)
(BLAST)
001: MGYWNSKVVP KFKKLFEKNS AKKAAAAEAT KTFDESKETI NKEIEEKKTE LQPKVVETYE ATSAEVKALV RDPKVAGLKK NSAAVQKYLE ELVKIEFPGS
101: KAVSEASSSF GAGYVAGPVT FIFEKVSVFL PEEVKTKEIP VEEVKAEEPA KTEEPAKTEG TSGEKEEIVE ETKKGETPET AVVEEKKPEV EEKKEEATPA
201: PAVVETPVKE PETTTTAPVA EPPKP
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)