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AT1G78870.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
golgi 1.000
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31615849 (2019): plastid plastid thylakoid
  • PMID:31520498 (2020): mitochondrion
  • PMID:25900983 (2015): Golgi
  • PMID:25900983 (2015): Golgi Golgi apparatus Golgi membrane
  • PMID:25900983 (2015): Golgi trans-Golgi network
  • PMID:25900983 (2015): Golgi trans-Golgi network early endosome
  • PMID:25900983 (2015): Golgi trans-Golgi network multivesicular body
  • PMID:25641898 (2015): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : ubiquitin-conjugating enzyme 35
Curator
Summary (TAIR10)
UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.
Computational
Description (TAIR10)
ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 10164 Blast hits to 10157 proteins in 398 species: Archae - 0; Bacteria - 2; Metazoa - 4429; Fungi - 2211; Plants - 1825; Viruses - 23; Other Eukaryotes - 1674 (source: NCBI BLink).
Protein Annotations
EnsemblPlants:AT1G78870EnsemblPlants:AT1G78870.1entrez:844224hmmpanther:PTHR24067
hmmpanther:PTHR24067:SF159Pfam:PF00179Pfscan:PS50127scanprosite:PS00183
tair10-symbols:UBC13Atair10-symbols:UBC35unipathway:UPA00143
Coordinates (TAIR10) chr1:+:29650589..29652203
Molecular Weight (calculated) 17348.90 Da
IEP (calculated) 8.44
GRAVY (calculated) -0.35
Length 154 amino acids
Sequence (TAIR10)
(BLAST)
001: MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE GRGVFKLELF LPEEYPMAAP KVRFLTKIYH PNIDKLGRIC LDILKDKWSP
101: ALQIRTVLLS IQALLSAPNP DDPLSENIAK HWKSNEAEAV DTAKEWTRLY ASGA
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)