AT4G20260.6
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Subcellular Consensus
(Prediction and Experimental) min:  :max.
SUBAcon:cytosol 0.991 What is SUBAcon? |
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| Experimental Localisations and PPI |
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SUBAcon links
AGI-AGI relationships |
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| Description (TAIR10) | protein_coding : plasma-membrane associated cation-binding protein 1 | ||||||||||||||||||||||||
| Curator Summary (TAIR10) |
Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels. | ||||||||||||||||||||||||
| Computational Description (TAIR10) |
plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469). | ||||||||||||||||||||||||
| Protein Annotations |
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| Coordinates (TAIR10) | chr4:+:10941593..10943227 | ||||||||||||||||||||||||
| Molecular Weight (calculated) | 18064.50 Da | ||||||||||||||||||||||||
| IEP (calculated) | 7.00 | ||||||||||||||||||||||||
| GRAVY (calculated) | -0.48 | ||||||||||||||||||||||||
| Length | 166 amino acids | ||||||||||||||||||||||||
| Sequence (TAIR10) (BLAST) |
001: MGYWNSKVVP KFKKLFEKNS AKKAAAAEAT KTFDESKETI NKEIEEKKTE LQPKVVETYE ATSAEVKALV RDPKVAGLKK NSAAVQKYLE ELVKIEFPGS 101: KAVSEASSSF GAGYVAGPVT FIFEKVSVFL PEEKKEEATP APAVVETPVK EPETTTTAPV AEPPKP |
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| See Also |
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Citation
If you find this resource useful please cite one of the following publications:
Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)
Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)