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AT4G15530.2
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
cytosol 0.500
plastid 0.500
ASURE: cytosol,plastid
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31520498 (2020): mitochondrion
  • PMID:24872594 (2014): plastid plastid stroma
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : pyruvate orthophosphate dikinase
Curator
Summary (TAIR10)
Encodes a dual-targeted protein believed to act as a pyruvate, orthophosphate dikinase. These enzymes are normally associated with C4 photosynthesis which does not occur in Arabidopsis. However, PPDK may play a role in remobilizing nitrogen during leaf senescence in Arabidopsis. The product of the long transcript (.1 gene model) was shown to be targeted to the chloroplast, whereas the shorter transcript (no targeting sequence) accumulates in the cytosol. The two proteins were also found to be expressed in slightly different tissues.
Computational
Description (TAIR10)
pyruvate orthophosphate dikinase (PPDK); FUNCTIONS IN: kinase activity, pyruvate, phosphate dikinase activity; INVOLVED IN: phosphorylation, response to absence of light; LOCATED IN: cytosol, nucleus, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: PEP-utilising enzyme, mobile domain (InterPro:IPR008279), ATP-grasp fold, subdomain 1 (InterPro:IPR013815), Pyruvate, phosphate dikinase (InterPro:IPR010121), Pyruvate/Phosphoenolpyruvate kinase, catalytic core (InterPro:IPR015813), Pyruvate phosphate dikinase, PEP/pyruvate-binding (InterPro:IPR002192), PEP-utilising enzyme, mobile region, conserved site (InterPro:IPR018274), ATP-grasp fold, subdomain 2 (InterPro:IPR013816), PEP-utilising enzyme (InterPro:IPR000121); Has 15214 Blast hits to 15125 proteins in 2575 species: Archae - 291; Bacteria - 9898; Metazoa - 12; Fungi - 19; Plants - 160; Viruses - 0; Other Eukaryotes - 4834 (source: NCBI BLink).
Protein Annotations
eggNOG:COG0574eggNOG:ENOG410IIM3EMBL:AK317578EnsemblPlants:AT4G15530
EnsemblPlants:AT4G15530.2entrez:827226ExpressionAtlas:B9DHM5Gene3D:3.20.20.60
Gene3D:3.30.1490.20Gene3D:3.30.470.20Gene3D:3.50.30.10GeneID:827226
GO:GO:0005524GO:GO:0006090GO:GO:0016301GO:GO:0046872
GO:GO:0050242Gramene:AT4G15530.2gramene_pathway:2.7.9.1gramene_pathway:PWY-6549
hmmpanther:PTHR22931hmmpanther:PTHR22931:SF9HOGENOM:HOG000039664InterPro:IPR000121
InterPro:IPR002192InterPro:IPR008279InterPro:IPR010121InterPro:IPR013815
InterPro:IPR013816InterPro:IPR015813InterPro:IPR018274InterPro:IPR023151
KEGG:00620+2.7.9.1KEGG:00710+2.7.9.1KEGG:00720+2.7.9.1PANTHER:PTHR22931:SF9
PaxDb:B9DHM5Pfam:PF00391Pfam:PF01326Pfam:PF02896
PIRSF:PIRSF000853PRIDE:B9DHM5PROSITE:PS00370PROSITE:PS00742
ProteinModelPortal:B9DHM5RefSeq:NP_193288.2scanprosite:PS00370scanprosite:PS00742
SMR:B9DHM5STRING:3702.AT4G15530.5SUPFAM:SSF51621SUPFAM:SSF52009
SUPFAM:SSF56059TAIR:AT4G15530tair10-symbols:PPDKTIGRfam:TIGR01828
TIGRFAMs:TIGR01828UniGene:At.23286UniGene:At.44851UniProt:B9DHM5
Coordinates (TAIR10) chr4:-:8864828..8869183
Molecular Weight (calculated) 95398.30 Da
IEP (calculated) 5.19
GRAVY (calculated) -0.14
Length 875 amino acids
Sequence (TAIR10)
(BLAST)
001: MMQRVFTFGK GRSEGNKGMK SLLGGKGANL AEMASIGLSV PPGLTISTEA CQQYQIAGKK LPEGLWEEIL EGLSFIERDI GASLADPSKP LLLSVRSGAA
101: ISMPGMMDTV LNLGLNDQVV VGLAAKSGER FAYDSFRRFL DMFGDVVMGI PHAKFEEKLE RMKERKGVKN DTDLSAADLK ELVEQYKSVY LEAKGQEFPS
201: DPKKQLELAI EAVFDSWDSP RANKYRSINQ ITGLKGTAVN IQCMVFGNMG DTSGTGVLFT RNPSTGEKKL YGEFLVNAQG EDVVAGIRTP EDLDTMKRFM
301: PEAYAELVEN CNILERHYKD MMDIEFTVQE ERLWMLQCRA GKRTGKGAVK IAVDMVGEGL VEKSSAIKMV EPQHLDQLLH PQFHDPSGYR EKVVAKGLPA
401: SPGAAVGQVV FTAEEAEAWH SQGKTVILVR TETSPDDVGG MHAAEGILTA RGGMTSHAAV VARGWGKCCI AGCSEIRVDE NHKVLLIGDL TINEGEWISM
501: NGSTGEVILG KQALAPPALS PDLETFMSWA DAIRRLKVMA NADTPEDAIA ARKNGAQGIG LCRTEHMFFG ADRIKAVRKM IMAVTTEQRK ASLDILLPYQ
601: RSDFEGIFRA MDGLPVTIRL LDPPLHEFLP EGDLDNIVHE LAEETGVKED EVLSRIEKLS EVNPMLGFRG CRLGISYPEL TEMQARAIFE AAASMQDQGV
701: TVIPEIMVPL VGTPQELGHQ VDVIRKVAKK VFAEKGHTVS YKVGTMIEIP RAALIADEIA KEAEFFSFGT NDLTQMTFGY SRDDVGKFLP IYLAKGILQH
801: DPFEVLDQQG VGQLIKMATE KGRAARPSLK VGICGEHGGD PSSVGFFAEA GLDYVSCSPF RVPIARLAAA QVVVA
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)