AT4G09020.1
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Subcellular Consensus
(Prediction and Experimental) min:  :max.
SUBAcon:plastid 1.000 ASURE: plastid What is SUBAcon? |
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| Experimental Localisations and PPI |
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SUBAcon links
AGI-AGI relationships |
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| Description (TAIR10) | protein_coding : isoamylase 3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Curator Summary (TAIR10) |
Encodes an isoamylase-like protein. Mutant studies show that the gene is strongly involved in starch breakdown. A GUS-protein fusion product was shown to localize to the surface of chloroplastic structures reminiscent of starch granules. In the mutants, the chloroplastic α-amylase AMY3 is upregulated. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Computational Description (TAIR10) |
isoamylase 3 (ISA3); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast, chloroplast stroma, chloroplast starch grain; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl hydrolase, family 13, all-beta (InterPro:IPR013780), Immunoglobulin-like fold (InterPro:IPR013783), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 1 (TAIR:AT2G39930.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Protein Annotations |
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| Coordinates (TAIR10) | chr4:+:5784099..5788839 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Molecular Weight (calculated) | 86326.10 Da | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| IEP (calculated) | 5.96 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GRAVY (calculated) | -0.47 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Length | 764 amino acids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sequence (TAIR10) (BLAST) |
001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK 101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG 201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR 301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC 401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK 501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI 601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD 701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK |
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| See Also |
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Citation
If you find this resource useful please cite one of the following publications:
Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)
Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)