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AT3G44300.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
cytosol 1.000
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31871212 (2020): mitochondrion
  • PMID:31520498 (2020): mitochondrion
  • PMID:31186290 (2019): cytosol None
  • PMID:31186290 (2019): plastid plastid stroma
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:30447334 (2019): plasma membrane
  • PMID:28865150 (2017): extracellular region plant-type cell wall
  • PMID:27122571 (2016): mitochondrion
  • PMID:26572690 (2016): extracellular region plant-type cell wall
  • PMID:23673981 (2013): plastid plastid stroma plastoglobules
  • PMID:22550958 (2012): plastid
  • PMID:21433285 (2011): plasma membrane
  • PMID:21166475 (2011): cytosol
  • PMID:19334764 (2009): plasma membrane
  • PMID:15215502 (2004): plant-type vacuole
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : nitrilase 2
Curator
Summary (TAIR10)
Encodes an enzyme that catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA) (nitrile aminohydrolase, EC 3.5.5.1) and IAN to indole-3-acetamide (IAM) at lower levels. Mutants have reduced sensitivity to IAN and are sensitive to IAA. This enzyme likely participates in other non-auxin-related metabolic pathways.
Computational
Description (TAIR10)
nitrilase 2 (NIT2); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: response to cadmium ion, indoleacetic acid biosynthetic process, response to bacterium; LOCATED IN: cellular_component unknown; EXPRESSED IN: cotyledon, cultured cell; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 1 (TAIR:AT3G44310.3); Has 7677 Blast hits to 7598 proteins in 1742 species: Archae - 157; Bacteria - 4958; Metazoa - 446; Fungi - 568; Plants - 325; Viruses - 11; Other Eukaryotes - 1212 (source: NCBI BLink).
Protein Annotations
eggNOG:COG0388eggNOG:KOG0805EMBL:AK226450EMBL:AL353865
EMBL:BT025235EnsemblPlants:AT3G44300EnsemblPlants:AT3G44300.1entrez:823555
ExpressionAtlas:Q1LYZ1Gene3D:3.60.110.10GeneID:823555GO:GO:0006807
GO:GO:0016810Gramene:AT3G44300.1gramene_plant_reactome:1119486gramene_plant_reactome:6876740
hmmpanther:PTHR23088hmmpanther:PTHR23088:SF31InterPro:IPR000132InterPro:IPR003010
KEGG:ath:AT3G44300KO:K01501OMA:PFNGVASPfam:P32962
Pfam:PF00795Pfscan:PS50263PhylomeDB:Q1LYZ1PROSITE:PS00920
PROSITE:PS00921PROSITE:PS50263RefSeq:NP_190016.1scanprosite:PS00920
scanprosite:PS00921SMR:Q1LYZ1STRING:3702.AT3G44300.1SUPFAM:SSF56317
TAIR:AT3G44300tair10-symbols:AtNIT2tair10-symbols:NIT2UniGene:At.5404
UniGene:At.67732UniProt:P32962UniProt:Q1LYZ1
Coordinates (TAIR10) chr3:+:15983351..15985172
Molecular Weight (calculated) 37155.10 Da
IEP (calculated) 5.24
GRAVY (calculated) -0.20
Length 339 amino acids
Sequence (TAIR10)
(BLAST)
001: MSTSENTPFN GVASSTIVRA TIVQASTVYN DTPATLEKAN KFIVEAASKG SELVVFPEAF IGGYPRGFRF GLGVGVHNEE GRDEFRKYHA SAIKVPGPEV
101: EKLAELAGKN NVYLVMGAIE KDGYTLYCTA LFFSPQGQFL GKHRKLMPTS LERCIWGQGD GSTIPVYDTP IGKLGAAICW ENRMPLYRTA LYAKGIELYC
201: APTADGSKEW QSSMLHIAIE GGCFVLSACQ FCLRKDFPDH PDYLFTDWYD DKEPDSIVSQ GGSVIISPLG QVLAGPNFES EGLITADLDL GDVARAKLYF
301: DSVGHYSRPD VLHLTVNEHP KKPVTFISKV EKAEDDSNK
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)