AT1G03310.1
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Subcellular Consensus
(Prediction and Experimental) min:  :max.
SUBAcon:plastid 1.000 ASURE: plastid What is SUBAcon? |
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| Experimental Localisations and PPI |
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SUBAcon links
AGI-AGI relationships |
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| Description (TAIR10) | protein_coding : debranching enzyme 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Curator Summary (TAIR10) |
Encodes a protein with strong similarity to isoamylase (EC:3.2.1.68) however lacks critical residues known to be important for activity. Appears to co localize with ISA1 in the chloroplast isoamylase complex. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. It has been postulated that AtISA2 interacts with AtISA1 to form the Iso1 complex. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Computational Description (TAIR10) |
debranching enzyme 1 (DBE1); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, amylopectin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 10041 Blast hits to 10011 proteins in 2123 species: Archae - 109; Bacteria - 8214; Metazoa - 136; Fungi - 197; Plants - 613; Viruses - 0; Other Eukaryotes - 772 (source: NCBI BLink). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Protein Annotations |
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| Coordinates (TAIR10) | chr1:+:813975..816623 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Molecular Weight (calculated) | 98889.00 Da | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| IEP (calculated) | 5.72 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GRAVY (calculated) | -0.27 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Length | 882 amino acids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sequence (TAIR10) (BLAST) |
001: MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF 101: RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL 201: KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR 301: YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL 401: KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL 501: NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN 601: FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG 701: DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP 801: NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA |
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| See Also |
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Citation
If you find this resource useful please cite one of the following publications:
Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)
Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)