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AT5G50950.2
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
cytosol 0.500
mitochondrion 0.500
ASURE: cytosol,mitochondrion
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:28865150 (2017): extracellular region plant-type cell wall
  • PMID:24727099 (2014): mitochondrion
  • PMID:23673981 (2013): plastid plastid stroma plastoglobules
  • PMID:21311031 (2011): mitochondrion
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : FUMARASE 2
Curator
Summary (TAIR10)
Encodes a fumarase enzyme initially shown to be in the mitochondria through proteomic studies but later shown to be present in the cytosol using an RFP fluorescent protein tag. It appears to be important for the accumulation of fumarate from malate in leaves in the light, and helps to promote nitrogen assimilation under high nitrogen conditions. It does not appear to be necessary for lipid metabolism and seedling growth.
Computational
Description (TAIR10)
FUMARASE 2 (FUM2); FUNCTIONS IN: fumarate hydratase activity, protein binding; INVOLVED IN: nitrate assimilation, response to salt stress, fumarate metabolic process; LOCATED IN: cytosol, mitochondrion; EXPRESSED IN: male gametophyte, guard cell, root, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Fumarate hydratase, class II (InterPro:IPR005677), L-Aspartase-like (InterPro:IPR008948), Lyase 1, N-terminal (InterPro:IPR022761), Fumarate lyase, conserved site (InterPro:IPR020557), Fumarase C, C-terminal (InterPro:IPR018951), Fumarate lyase (InterPro:IPR000362); BEST Arabidopsis thaliana protein match is: fumarase 1 (TAIR:AT2G47510.2); Has 20242 Blast hits to 20233 proteins in 2834 species: Archae - 403; Bacteria - 13389; Metazoa - 329; Fungi - 314; Plants - 117; Viruses - 0; Other Eukaryotes - 5690 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT5G50950-MONOMERBioCyc:ARA:GQT-2362-MONOMERBioCyc:ARA:GQT-2363-MONOMERBioCyc:MetaCyc:AT5G50950-MONOMER
BioGrid:20414BRENDA:4.2.1.2EC:4.2.1.2eggNOG:COG0114
eggNOG:KOG1317EMBL:AB017063EMBL:AY052197EMBL:AY072393
EMBL:AY113070EMBL:CP002688EnsemblPlants:AT5G50950EnsemblPlants:AT5G50950.2
entrez:835168ExpressionAtlas:Q9FI53Gene3D:1.10.275.10GeneID:835168
Genevisible:Q9FI53GO:GO:0004333GO:GO:0005739GO:GO:0005829
GO:GO:0006099GO:GO:0006106GO:GO:0006108GO:GO:0009507
GO:GO:0009651GO:GO:0042128GO:GO:0045239GO:GO:0051262
gramene_pathway:4.2.1.2gramene_pathway:PWY-561gramene_pathway:PWY-5690gramene_pathway:PWYQT-4481
gramene_plant_reactome:1119533gramene_plant_reactome:6876687HAMAP:MF_00743hmmpanther:PTHR11444
hmmpanther:PTHR11444:SF1HOGENOM:HOG000061736InParanoid:Q9FI53IntAct:Q9FI53
InterPro:IPR000362InterPro:IPR005677InterPro:IPR008948InterPro:IPR018951
InterPro:IPR020557InterPro:IPR022761InterPro:IPR024083KEGG:00020+4.2.1.2
KEGG:00620+4.2.1.2KEGG:00720+4.2.1.2OMA:IGHHTAIPANTHER:PTHR11444
PaxDb:Q9FI53Pfam:PF00206Pfam:PF10415Pfam:Q9FI53
PhylomeDB:Q9FI53PRIDE:Q9FI53PRINTS:PR00149PRO:PR:Q9FI53
PROSITE:PS00163ProteinModelPortal:Q9FI53Proteomes:UP000006548Reactome:R-ATH-71403
RefSeq:NP_199908.1RefSeq:NP_851166.1scanprosite:PS00163SMR:Q9FI53
STRING:3702.AT5G50950.1SUPFAM:SSF48557TAIR:AT5G50950tair10-symbols:FUM2
TIGRfam:TIGR00979TIGRFAMs:TIGR00979UniGene:At.6963UniPathway:UPA00223
UniProt:Q9FI53
Coordinates (TAIR10) chr5:+:20729687..20733636
Molecular Weight (calculated) 54086.60 Da
IEP (calculated) 6.28
GRAVY (calculated) -0.25
Length 499 amino acids
Sequence (TAIR10)
(BLAST)
001: MAALTMQFEG EKKNVSEVAD VTLKQEDEQQ ERRSYSTPFR EERDTFGPIQ VPSDKLWGAQ TQRSLQNFEI GGDRERMPEP IVRAFGVLKK CAAKVNMEYG
101: LDPMIGEAIM EAAQEVAEGK LNDHFPLVVW QTGSGTQSNM NANEVIANRA AEILGHKRGE KIVHPNDHVN RSQSSNDTFP TVMHIAAATE ITSRLIPSLK
201: NLHSSLESKS FEFKDIVKIG RTHTQDATPL TLGQEFGGYA TQVEYGLNRV ACTLPRIYQL AQGGTAVGTG LNTKKGFDVK IAAAVAEETN LPFVTAENKF
301: EALAAHDACV ETSGSLNTIA TSLMKIANDI RFLGSGPRCG LGELSLPENE PGSSIMPGKV NPTQCEALTM VCAQVMGNHV AVTIGGSNGH FELNVFKPVI
401: ASALLHSIRL IADASASFEK NCVRGIEANR ERISKLLHES LMLVTSLNPK IGYDNAAAVA KRAHKEGCTL KHAAMKLGVL TSEEFDTLVV PEKMIGPSD
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)