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AT4G25000.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
extracellular 1.000
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31673584 (2019): extracellular region plant-type cell wall
  • PMID:30783145 (2019): extracellular region apoplast
  • PMID:30783145 (2019): extracellular region plant-type cell wall
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : alpha-amylase-like
Curator
Summary (TAIR10)
Predicted to be secreted protein based on signalP prediction. Involved in starch mobilization. Mutants are defective in alpha-amylase activity. (Note: AMY1 has been found in the literature to be referred to as AMY3, which is not to be confused with AMY3/At1g69830).
Computational
Description (TAIR10)
alpha-amylase-like (AMY1); FUNCTIONS IN: alpha-amylase activity; INVOLVED IN: response to gibberellin stimulus, response to abscisic acid stimulus; LOCATED IN: extracellular region, apoplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 13 (InterPro:IPR006046), Alpha-amylase, plant (InterPro:IPR013775), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047), Alpha-amylase, C-terminal beta-sheet (InterPro:IPR012850); BEST Arabidopsis thaliana protein match is: alpha-amylase-like 2 (TAIR:AT1G76130.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT4G25000-MONOMERCAZy:GH13EC:3.2.1.1eggNOG:COG0366
eggNOG:KOG0471EMBL:AL035523EMBL:AL161562EMBL:AY065233
EMBL:AY087021EMBL:AY117294EMBL:CP002687EnsemblPlants:AT4G25000
EnsemblPlants:AT4G25000.1entrez:828603Gene3D:2.60.40.1180Gene3D:3.20.20.80
GeneID:828603Genevisible:Q8VZ56GO:GO:0004556GO:GO:0005509
GO:GO:0005576GO:GO:0005975GO:GO:0009737GO:GO:0009739
GO:GO:0048046Gramene:AT4G25000.1gramene_pathway:3.2.1.1gramene_pathway:PWY-842
hmmpanther:PTHR10357hmmpanther:PTHR10357:SF153HOGENOM:HOG000239525InParanoid:Q8VZ56
InterPro:IPR006046InterPro:IPR006047InterPro:IPR012850InterPro:IPR013775
InterPro:IPR013780InterPro:IPR013781InterPro:IPR015902InterPro:IPR017853
KEGG:00500+3.2.1.1KEGG:ath:AT4G25000KO:K01176OMA:GEQGYMP
PANTHER:PTHR10357PaxDb:Q8VZ56Pfam:PF00128Pfam:PF07821
Pfam:Q8VZ56PhylomeDB:Q8VZ56PIR:T05521PIRSF:PIRSF001028
PRIDE:Q8VZ56PRINTS:PR00110PRO:PR:Q8VZ56ProMEX:Q8VZ56
ProteinModelPortal:Q8VZ56Proteomes:UP000006548RefSeq:NP_567714.1SMART:SM00642
SMART:SM00810SMR:Q8VZ56STRING:3702.AT4G25000.1SUPFAM:SSF51011
SUPFAM:SSF51445TAIR:AT4G25000tair10-symbols:AMY1tair10-symbols:ATAMY1
TMHMM:TMhelixUniGene:At.28556UniProt:Q8VZ56
Coordinates (TAIR10) chr4:-:12852109..12853825
Molecular Weight (calculated) 47380.00 Da
IEP (calculated) 5.82
GRAVY (calculated) -0.31
Length 423 amino acids
Sequence (TAIR10)
(BLAST)
001: MTSLHTLLFS SLLFFIVFPT FTFSSTLLFQ SFNWESWKKE GGFYNSLHNS IDDIANAGIT HLWLPPPSQS VAPEGYLPGK LYDLNSSKYG SEAELKSLIK
101: ALNQKGIKAL ADIVINHRTA ERKDDKCGYC YFEGGTSDDR LDWDPSFVCR NDPKFPGTGN LDTGGDFDGA PDIDHLNPRV QKELSEWMNW LKTEIGFHGW
201: RFDYVRGYAS SITKLYVQNT SPDFAVGEKW DDMKYGGDGK LDYDQNEHRS GLKQWIEEAG GGVLTAFDFT TKGILQSAVK GELWRLKDSQ GKPPGMIGIM
301: PGNAVTFIDN HDTFRTWVFP SDKVLLGYVY ILTHPGTPCI FYNHYIEWGL KESISKLVAI RNKNGIGSTS SVTIKAAEAD LYLAMIDDKV IMKIGPKQDV
401: GTLVPSNFAL AYSGLDFAVW EKK
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)