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AT5G25880.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
cytosol 1.000
ASURE: cytosol
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31871212 (2020): mitochondrion
  • PMID:31023727 (2019): mitochondrion
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:30447334 (2019): plasma membrane
  • PMID:24361574 (2014): plastid plastid stroma
  • PMID:19334764 (2009): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : NADP-malic enzyme 3
Curator
Summary (TAIR10)
The malic enzyme (EC 1.1.1.40) encoded by the ATNADP-ME3 is presumably cytosolic and restricted in its expression by both developmental and cell-specific signals.
Computational
Description (TAIR10)
NADP-malic enzyme 3 (NADP-ME3); FUNCTIONS IN: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity, oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor, malic enzyme activity; INVOLVED IN: N-terminal protein myristoylation, malate metabolic process, protein homooligomerization; LOCATED IN: cytosol; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, C globular stage, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NADP-malic enzyme 2 (TAIR:AT5G11670.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT5G25880-MONOMERBioGrid:17932BRENDA:1.1.1.40EC:1.1.1.40
eggNOG:COG0281eggNOG:KOG1257EMBL:AF149413EMBL:CP002688
EnsemblPlants:AT5G25880EnsemblPlants:AT5G25880.1entrez:832657Gene3D:3.40.50.10380
Gene3D:3.40.50.720GeneID:832657Genevisible:Q9XGZ0GO:GO:0004471
GO:GO:0004473GO:GO:0005829GO:GO:0006108GO:GO:0008948
GO:GO:0046872GO:GO:0051260GO:GO:0051287Gramene:AT5G25880.1
hmmpanther:PTHR23406hmmpanther:PTHR23406:SF31HOGENOM:HOG000042486InParanoid:Q9XGZ0
InterPro:IPR001891InterPro:IPR012301InterPro:IPR012302InterPro:IPR015884
InterPro:IPR016040KEGG:ath:AT5G25880KO:K00029OMA:SYRWPED
PaxDb:Q9XGZ0Pfam:PF00390Pfam:PF03949Pfam:Q9XGZ0
PhylomeDB:Q9XGZ0PIRSF:PIRSF000106PRIDE:Q9XGZ0PRINTS:PR00072
PRO:PR:Q9XGZ0PROSITE:PS00331ProteinModelPortal:Q9XGZ0Proteomes:UP000006548
RefSeq:NP_197960.1SABIO-RK:Q9XGZ0scanprosite:PS00331SMART:SM00919
SMART:SM01274SMR:Q9XGZ0STRING:3702.AT5G25880.1SUPFAM:SSF51735
SUPFAM:SSF53223TAIR:AT5G25880tair10-symbols:ATNADP-ME3tair10-symbols:NADP-ME3
UniGene:At.43327UniProt:Q9XGZ0
Coordinates (TAIR10) chr5:+:9024549..9028260
Molecular Weight (calculated) 64613.90 Da
IEP (calculated) 6.99
GRAVY (calculated) -0.13
Length 588 amino acids
Sequence (TAIR10)
(BLAST)
001: MGTNQTQISD EYVTGNSSGV GGGISDVYGE DSATLDQLVT PWVTSVASGY TLMRDPRYNK GLAFTDKERD AHYITGLLPP VVLSQDVQER KVMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI YRRPQGLYIS LKEKGKILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNNE FYIGLKQKRA NGEEYAEFLQ EFMCAVKQNY GEKVLVQFED FANHHAFELL SKYCSSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKSLADHT FLFLGAGEAG TGIAELIALK ISKETGKPID ETRKKIWLVD SKGLIVSERK ESLQHFKQPW AHDHKPVKEL
401: LAAVNAIKPT VLIGTSGVGK TFTKEVVEAM ATLNEKPLIL ALSNPTSQAE CTAEEAYTWT KGRAIFASGS PFDPVQYDGK KFTPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL ASQVTEENFA NGLIYPPFAN IRKISANIAA SVGAKTYELG LASNLPRPKD LVKMAESCMY SPVYRNFR
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)