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AT1G09100.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
cytosol 0.500
nucleus 0.500
ASURE: cytosol,nucleus
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31541795 (2020): plasma membrane
  • PMID:31520498 (2020): mitochondrion
  • PMID:30961429 (2019): nucleus
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:30447334 (2019): plasma membrane
  • PMID:29104584 (2017): nucleus nuclear matrix nucleolus
  • PMID:27177187 (2016): nucleus
  • PMID:23673981 (2013): plastid plastid stroma plastoglobules
  • PMID:22550958 (2012): plastid
  • PMID:19334764 (2009): plasma membrane
  • PMID:17317660 (2007): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : 26S proteasome AAA-ATPase subunit RPT5B
Curator
Summary (TAIR10)
Encodes RPT5b (Regulatory Particle 5b), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
Computational
Description (TAIR10)
26S proteasome AAA-ATPase subunit RPT5B (RPT5B); FUNCTIONS IN: ATPase activity, calmodulin binding; INVOLVED IN: glucose mediated signaling pathway, proteasomal protein catabolic process, embryo sac development, pollen development; LOCATED IN: proteasome complex, plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: regulatory particle triple-A ATPase 5A (TAIR:AT3G05530.1); Has 33470 Blast hits to 31156 proteins in 3185 species: Archae - 1434; Bacteria - 12401; Metazoa - 4942; Fungi - 3663; Plants - 3251; Viruses - 53; Other Eukaryotes - 7726 (source: NCBI BLink).
Protein Annotations
BioGrid:22672eggNOG:COG1222eggNOG:KOG0652EMBL:AC000106
EMBL:AF412095EMBL:AY050423EMBL:AY124813EMBL:CP002684
EMBL:EU980096EnsemblPlants:AT1G09100EnsemblPlants:AT1G09100.1entrez:837431
Gene3D:3.40.50.300GeneID:837431Genevisible:O04019GO:GO:0000502
GO:GO:0005524GO:GO:0005886GO:GO:0008540GO:GO:0009553
GO:GO:0009555GO:GO:0010255GO:GO:0010498GO:GO:0016020
GO:GO:0017025GO:GO:0030433GO:GO:0031595GO:GO:0031597
GO:GO:0036402GO:GO:0045899GO:GO:1901800Gramene:AT1G09100.1
hmmpanther:PTHR23073hmmpanther:PTHR23073:SF7HOGENOM:HOG000225143InParanoid:O04019
IntAct:O04019InterPro:IPR003593InterPro:IPR003959InterPro:IPR003960
InterPro:IPR005937InterPro:IPR027417iPTMnet:O04019KEGG:ath:AT1G09100
KO:K03065ncoils:CoilOMA:KMNVNADPaxDb:O04019
Pfam:O04019Pfam:PF00004PhylomeDB:O04019PIR:C86223
PRIDE:O04019PRO:PR:O04019PROSITE:PS00674ProteinModelPortal:O04019
Proteomes:UP000006548Reactome:R-ATH-1236978Reactome:R-ATH-174184Reactome:R-ATH-349425
Reactome:R-ATH-5632684Reactome:R-ATH-68949Reactome:R-ATH-69017Reactome:R-ATH-983168
RefSeq:NP_172384.1scanprosite:PS00674SMART:SM00382SMR:O04019
STRING:3702.AT1G09100.1SUPFAM:SSF52540TAIR:AT1G09100tair10-symbols:RPT5B
TIGRfam:TIGR01242TIGRFAMs:TIGR01242UniGene:At.10379UniGene:At.42241
UniProt:O04019
Coordinates (TAIR10) chr1:-:2936675..2939258
Molecular Weight (calculated) 47040.30 Da
IEP (calculated) 4.63
GRAVY (calculated) -0.39
Length 423 amino acids
Sequence (TAIR10)
(BLAST)
001: MATAMAEDTS FEGDQLASMT TDDIGRASRL LANEIRILKE ESQRTNLDLE SVKEKIKENQ EKIKLNKQLP YLVGNIVEIL EMSPEDDAEE DGANIDLDSQ
101: RKGKCVVLKT STRQTIFLPV VGLVDPDTLK PGDLVGVNKD SYLILDTLPS EYDSRVKAME VDEKPTEDYN DIGGLEKQIQ ELVEAIVLPM THKEQFEKLG
201: IRPPKGVLLY GPPGTGKTLM ARACAAQTNA TFLKLAGPQL VQMFIGDGAK LVRDAFLLAK EKSPCIIFID EIDAIGTKRF DSEVSGDREV QRTMLELLNQ
301: LDGFSSDDRI KVIAATNRAD ILDPALMRSG RLDRKIEFPH PTEEARGRIL QIHSRKMNVN ADVNFEELAR STDDFNGAQL KAVCVEAGML ALRRDATEVN
401: HEDFNEGIIQ VQAKKKASLN YYA
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)